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第24号公告板 - Spring 2006
VirologyPMWS-Circovirus-PDNS
GRASLAND B, BLANCHARD P, OGER A, BIGARRE L, CARIOLET R, JESTIN A
Impact of the route on the transfection effectiveness with infectious cloned genomic DNA of type 2 porcine circovirus.
International Conference on Animal Circoviruses and Associated Diseases, European Society for Veterinary Virology, 2005
The transfection effectiveness of a cloned porcine circovirus type 2 (PCV2) inoculated by the natural transmission routes of the virus was investigated. Thirty-five specific-pathogen-free piglets (7 weeks old) were randomly divided into 4 groups. All piglets were inoculated with 400?g of PCV2 genomic DNA either intramuscularly - IM (Group 2), intratracheally - IT (Group 3) or oro-nasally - ON (Group 4). Group 1 was the control group. The experiment lasted 34 days following transfection. The seroconversion to PCV2 antibodies was determined by means of an ELISA test. A real time quantitative PCR was used to quantify the PCV2 genomes and an IPMA was used to quantify PCV2 infectious particles. The results showed that the PCV2 genomic DNA induced the development of PCV2 particles, but that the transfection was more efficient when the PCV2 genomic DNA was administered intramuscularly.
