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第21号公告板 - Spring 2005
VirologyPorcine Reproductive and Respiratory Syndrome (PRRS)
DELPUTTE PL, MEERTS P, COSTERS S, NAUWYNCK HJ
Effect of virus-specific antibodies on attachment, internalization and infection of porcine reproductive and respiratory syndrome virus in primary macrophages.
Veterinary Immunology and Immunopathology, 2004, Volume 102, Nº3, 179-188
Evidence of differences between PRRSV infection of primary macrophages and PRRSV infection of Marc-145 cells used so far for the detection of virus neutralizing (VN) antibodies has recently been pointed out. As a consequence, it cannot be stated that high VN antibody titers, as determined by serum neutralization on Marc-145 cells, protect primary macrophages against infection. The effect of VN antibodies on infection of primary macrophages was thus investigated. PRRSV-negative pigs were inoculated with Lelystad virus (LV), blood samples were then collected at regular intervals and VN antibodies at 14 and 72 days post inoculation (DPI) were obtained by purification. VN antibodies obtained from LV infected pigs at 72 DPI blocked the infection of macrophages with LV, but provided partial protection against an homologous strain of PRRSV and no protection against an heterologous strain. VN antibodies from LV infected pigs were shown to have an effect on LV attachment and internalization. At 14 DPI, VN antibodies from LV infected pigs recognized the LV N protein while at 72 DPI VN antibodies further recognized the two major envelope proteins M and GP5. When tested with the homologous strain, VN antibodies from LV infected pigs recognized the N and M proteins nut not the GP5 protein.
