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第19号公告板 - Fall 2004
VirologyPMWS-Circovirus-PDNS
WANG C, HUANG TS, HUANG CC, TU C, JONG MH, LIN SY, LAI SS
Characterization of porcine circovirus type 2 in Taiwan
Journal of Veterinary Medical Science, 2004, Volume 66, N?5, 469-475
Routine PCR for PCV2 detection was applied to tissue samples collected from 623 Taiwanese diseased pigs over a 2-year period (2000-2002). The clinical conditions of the diseased pigs included PMWS, abortion, nervous disorders, sudden death, PDNS and non-specific clinical signs. PCV2 was detected in 49.6% of the cases. PCV2 infection may thus be present in pigs showing a large variety of clinical signs. A total of 8 PCV2 isolates were sequenced (4 from PMWS-affected pigs, 2 from PDNS-affected pigs, 1 from a pig with nervous disorders, 1 from a case of abortion) and were shown to share 95-99% homology. A further comparison of these isolates with 6 other isolates from the USA, Canada, Spain, Germany, Korea and Japan, revealed that all shared 92-99% homology. The phylogenetic analysis of the 8 Taiwanese isolates together with 32 PCV2 isolates from various parts of the world allowed to classify them into one large group containing two minor subgroups, the Taiwanese isolates being present in both subgroups. In addition, a large-scale serological survey revealed that about 83.5% of the Taiwanese pig population was seropositive to PCV2 suggesting that PCV2 infection is widely present in Taiwanese pig herds.
