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第17号公告板 - Winter 2003
VirologyPorcine Reproductive and Respiratory Syndrome (PRRS)
TORRISON J, ROSSOW K, YESKE P
Use of PRRS virus sequence information within herds.
Allen D. Leman Swine Conference 2003: 46-51
BO98439
The control of porcine reproductive and respiratory syndrome virus (PRRSV) is still under investigation. The determination and interpretation of its genomic sequence represents a useful weapon to battle against it. The recommended sampling protocol during an outbreak includes serum samples from clinically infected pigs (10-15 piglets at 4-5 days of age and 10-15 sows). Then samples are amplified by PCR and the sequence of the virus is determined with a comprehensive cost of 195$ for each sample. The interpretation of the virus sequence is based on three diagnostic reports i.e. the sequence of the opening reading frame 5 region, the dendrogram (relationships among groups of viruses) and the homology chart (percent base similarities of virus sequences). In PRRSV-positive herds, in case of concurrent infection, it is possible to determine whether the virus originates from the indigenous virus by mutation or whether it results from outside sources.
